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Cell culture and transfection Neuro2A cells were grown in a 1:1 mixture of DMEM and Opti-MEM, supplemented with 5% fetal bovine serum, 100 U/ml penicillin, and 100 g/ml streptomycin

Patient-Reported Outcomes: Quality of Life Questionnaires : Surveys such as the Thyroid-Related Quality of Life Patient-Reported Outcome (ThyPRO) to measure the impact of the disease and treatment on daily living [43-45]

Protein oxidation occurs when ROS modifies amino acid side chains, resulting in changes to protein structure and function

The results indicate that quercetin has a potential beneficial effect on T2DM, and functions by inhibiting pancreas cells ferroptosis, highlighting the promising curative effect of quercetin in T2DM [106]

Among these are neurodegenerative diseases, which are characterized by progressive neuronal impairment or loss leading to cognitive and motor decline (Wu et al., 2018)